Exposure to cetyl pyridinium chloride and loss of integrity of cell wall of mycobacteria.

Gomathi, S.; Kumar, V.; Gomathi , N.S. ; Selvakumar, N.

Indian Journal of Tuberculosis; 2013; 60; 223-226.

Summary: Background : Cetyl pyridinium chloride (CPC) liquefied sputum was shown to reduce AFB smear positivity presumably damaging cell wall of M. tuberculosis.

Settings : National Institute for Research in Tuberculosis, Chennai, (Tamil Nadu).

Objective : To assess the cell wall damage of mycobacteria in CPC liquefied sputum, by Transmission Electron Microscopy (TEM) and mycobacteriophage adsorption studies.

Methods : Pooled sputum sample from smear positive pulmonary TB patients was homogenized and liquefied with CPC. It was examined in TEM daily for four days, to assess cell wall damage of M. tuberculosis , and photomicrographs were taken. M. smegmatis mc 2 155, treated with CPC, was infected with mycobacteriophage (phAE129) to study phage adsorption on cell wall and plaque formation. CPC untreated sputum and M. smegmatis formed controls.

Results : Photomicrographs showed that cell wall of M. tuberculosis was intact in controls and damaged in CPC preserved sputum for 96 hours. Plaque formation was seen and absent respectively in CPC untreated and treated M. smegmatis cells.

Conclusion : Exposure to CPC damaged the cell wall of M. tuberculosis within 96 hours . Mycobacteriophage failed to form plaques after M. smegmatis mc 2 155 was treated with CPC implying inhibition of phage adsorption on damaged cell wall and thus providing a clue for poor staining and smear positivity in microscopy.

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