Anti-tubercular activity on leaves and roots of Sida rhombifolia L.

Papitha, N.; Jayshree, N.; Prabu Seenivasan, S.; Kumar, V.

International Journal of Pharmaceutical Sciences Review and Research; 2013; 20; 135-137.

Abstract: Sida rhombifolia L. is a very small perennial or annual plant which grows in tropical and subtropical areas. It belongs to the family Malvaceae. It grows upto 50-120 cm in height. It is commonly called Bala or Atibala. Modern research carried out on the Malvaceae plants reveals that most of the plants belonging to this family are medicinally important as they contain biologically active compounds. It has been reported for the presence of steroids, flavanoids, terpenoids, alkaloids, phenol, saponins, glycosides and tannins. Its leaves, stem, bark and roots are the usable parts. The present study was carried out to find out the susceptibility of Mycobacterium tuberculosis to ethyl acetate and ethanolic extracts of leaves and roots of Sida rhombifolia L. Luciferase reporter phage (LRP) assay was used to study the inhibition of Mycobacterium tuberculosis . The LRP assay showed that ethyl acetate extracts of leaves and roots at concentrations of 100 and 500 µg/ml showed good activity against the Standard strain of M. tuberculosis H37RV and clinical isolate of M. tuberculosis resistant to S, H, R and E. The results show that ethyl acetate leaf extract at 100 µg/ml was found to cause 67.18 % reduction in Relative Light Units (RLU) and 500 µg/ml was found to produce 83.61 % reduction in RLU. This shows that the ethyl acetate has anti tubercular activity against Mycobacterium tuberculosis . The study can further be extrapolated to isolate and identify the compound responsible for the anti tubercular activity.

Keywords: Sida rhombifolia L.; Luciferase reporter phage (LRP); Ethyl acetate and Ethanol extract; Anti tubercular activity

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