A selective and sensitive high performance liquid chromatography assay for the determination of cycloserine in human plasma.


Hemanth Kumar, A.K.; Polisetty, A.K.; Sudha, V.; Vijayakumar, A.; Ramachandran, G.


Indian Journal of Tuberculosis; 2018; 65(2): 118-123.


Abstract: Background: Cycloserine (CYC) is a second line antitubercular drug that is used for the treatment of multidrug resistant tuberculosis (MDR-TB) along with other antitubercular agents and is often used in developing countries. Monitoring CYC levels in plasma could be useful in the clinical management of patients with MDR-TB. A high performance liquid chromatography method for the determination of CYC in human plasma was developed.


Methods: The method involved extraction of the sample using solid phase extraction cartridges and analysis of the extracted sample using a reverse phase T3 column (150 mm) and detection at 240 nm with Photo Diode Array (PDA) detector. The chromatogram was run for 15 min at a ?ow rate of 0.4 ml/min at 30 8C.


Results and conclusion: The assay was speci?c for CYC and linear from 5.0 to 50.0 mg/ml. The relative standard deviations of within- and between-day assays were less than 10%. Recovery of CYC ranged from 102% to 109%. The interference of other second line anti-TB drugs in the assay of CYC was ruled out. The assay spans the concentration range of clinical interest. The speci?city and sensitivity of this assay makes it highly suitable for pharmacokinetic studies.


Keywords: HPLC; Cycloserine; Plasma; Tuberculosis; Antibiotics


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