Requirement of the mymA operon for appropriate cell wall ultrastructure and persistence of Mycobacterium tuberculosis in the spleens of guinea pigs.

Singh, A.; Gupta, R.; Vishwakarma, R.A.; Narayanan, P.R.; Paramasivan, C.N.; Ramanathan, V.D.; Tyagi, A.K.

Journal of Bacteriology, 2005; 187; 4173-4186.

Abstract: We had recently reported that the mymA operon (Rv3083 to Rv3089) of Mycobacterium tuberculosis is regulated by AraC/XylS transcriptional regulator VirS (Rv3082c) and is important for the cell envelope of M. tuberculosis . In this study, we further show that a virS mutant (Mtb ? virS ) and a mymA mutant (Mtb mym :: hyg ) of M. tuberculosis exhibit reduced contents and altered composition of mycolic acids along with the accumulation of saturated C 24 and C 26 fatty acids compared to the parental strain. These mutants were markedly more susceptible to major antitubercular drugs at acidic pH and also showed increased sensitivity to detergent (sodium dodecyl sulfate) and to acidic stress than the parental strain. We show that disruption of virS and mymA genes impairs the ability of M. tuberculosis to survive in activated macrophages, but not in resting macrophages, suggesting the importance of the mymA operon in protecting the bacterium against harsher conditions. Infection of guinea pigs with Mtb ? virS , Mtb mym :: hyg , and the parental strain resulted in an ~ 800-fold-reduced bacillary load of the mutant strains compared with the parental strain in spleens, but not in the lungs, of animals at 20 weeks postinfection. Phenotypic traits were fully complemented upon reintroduction of the virS gene into Mtb ? virS . These observations show the important role of the mymA operon in the pathogenesis of M. tuberculosis at later stages of the disease.

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