Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid.

Gurumurthy, P.; Gayathri, T.N.; Bhagavathy, S.; Venkatesan, P.

Indian Journal of Experimental Biology; 2004; 42; 68-73.

Abstract: A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 m g/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of atleast one week in urine stored at -20 o C.

Keywords: Ethambutol, Amberlite CG-50, Rifampicin, Isoniazid, Pyrazinamide

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