Smear examination of two specimens for diagnosis of pulmonary tuberculosis in Tiruvallur District, south India.

Gopi, P.G.; Subramani, R.; Selvakumar, N.; Santha, T.; Eusuff, S.I.; Narayanan, P.R.

International Journal of Tubercle and Lung Disease; 2004; 8; 824-828.

Summary: Settings: A general hospital and three peripheral health institutions (PHI) in Tiruvallur District, south India.

Objective: To validate the case detection strategy for diagnosis of pulmonary tuberculosis by smear microscopy of two sputum specimens versus three.

Methods: In the Revised National Tuberculosis Control Programme (RNTCP), three smears from every symptomatic patient attending the PHI were examined for acid-fast bacilli (AFB) by Ziehl-Neelsen (ZN) microscopy. The data from the Tuberculosis Laboratory Registers from the above centres were analysed.

Results: Of 7843 chest symptomatics who had provided three sputum specimens for examination, 895 (11.4%) were smear-positive on at least two specimens. Examination of two specimens – first spot and early morning specimens (837, 93.5%) or early morning and second spot specimens (843, 94.2%) – yielded the highest number of cases.

Conclusion: The examination of two sputum smears (one spot and an early morning) is as effective as examination of three smears.

Keywords: DOTS; RNTCP; sputum smear examination; AFB

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