Observations on the cultivation of M. leprae and M. tuberculosis in medium 'V' and 'V 1'.
Daniel Herbert; Prabhakar, R.
Indian Journal of Leprosy; 1992; 64(3); 341-347.
Skin scrapings from five different active sites were collected from 14 leprosy patients and inoculatd into medium V. Skin scrapings from three leprosy patients were inoculated into medium V1. All the cultures were incubated at 8-10°C. M. tuberculosis H 37 Rv, pretreatment isolates and streptomycin resistant strains were inoculated into medium V, with and without antibiotics, and incubated at 8-10°C as well as 37°C. Smears were made from the M. leprae and M. tuberculosis cultures at 0 hours and at different time points. The number of bacilli in the smears were counted. There was no increase in the number of M. leprae or M. tuberculosis in any of the cultures.
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