Development of DNA probes for M. tuberculosis.
Sujatha Narayanan; Sahadevan, B.; Ramanujam, S.; Prabhakar, R.;
Narayanan, P.R.
Indian Journal of Tuberculosis; 1992; 39; 99-105.
Attempts were made to develop DNA probes for M. tuberculosis . Random library of M. tuberculosis was constructed in plasmid pGEM -4. Selection of recombinant clones was made by hybridisation with 32 P labelled M. tuberculosis probe. Ten recombinant clones were selected on the basis of strong signals from the random library. These 10 clones named P TRC 1-10 were subjected to tests for specificity and sensitivity. On this basis, P TRC 4 was chosen and this is also useful in restriction fragment length polymorphism (RFLP) studies.
Back to List of publications / Home |