Monoclonal antibodies against Mycobacterium avium/intracellulare.

Paramasivan, C.N.; Paul Jackett, S.; Anthony Coates, R.M.; Douglas Lowrie, B.; Mitchison, D.A.

Indian Journal of Medical Research; 1988; 88; 13-17.

Ten hybridoma cell lines producing monoclonal antibodies (Mabs) against M. avium/intracellulare (Mai) serotype 8 were raised by the fusion of BALB/c mouse myecloma cells (SP2) to spleen cells from immunized BALB/c mice. The specificity of the monoclonal antibodies was defining using their differing abilities to bind to sonicates from a range of mycobacterial species and strains. The Mabs showed strain and species specificity. Three Mabs bound only to Mai serotype 8 and 1 Mab bound only to Mai serotypes 8 and 16, the only serotypes tested. The results indicate that Mabs specific for Mai species and serotypes can be produced. These could be useful for serodiagnostic and for epidemiological purposes.


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