Expression of the herpes simplex virus type 1 glycoprotein C gene requires sequences in the 5' noncoding region of the gene.

Jerry Weir, P.; Narayanan, P.R.

Journal of Virology; 1990; 64; 445-449.

The role of the 5' noncoding region of the herpes simplex virus type 1 glycoprotein C gel gene in viral gene expression was investigated with recombinant herpes viruses that contained the bacterial 3-galactosidase gene under the control of the gC promoter-regulatory region. Each of these viruses had the same DNA sequences from the start of gC transcription upstream to -114 but had variable segments of the downstream 140-base pair sequence that is between the start of gC transcription and translation. Analyses of ß-galactosidase expression and mRNA synthesis from these viruses demonstrated the importance of DNA sequences from the start of gC transcription downstream to +38 for optimal expression from the gC promoter.

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